Supplemental Materials for:

Matias, M.G., Gomolplitinant, K., Tamang, D.G. and Saier, M. H.

Animal Ca2+ Release-activated Ca2+ (CRAC) Channels are Homologous to and Derived
from the Ubiquitous Cation Diffusion Facilitators.


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SUPPLEMENTARY TABLES & FIGURES


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Figures:

Figure S1A: Multiple alignment of Orai protein homologues.

Figure S1B: Multiple alignment of Stim protein homologues.

Figure S1C: Multiple alignment of CDF protein homologues.

Figure S2A: Phylogenetic tree of Orai proteins. Protein abbreviations are as indicated in table S1. Clusters are labeled 1-6.

Figure S2B: Phylogenetic tree of Stim proteins. Protein abbreviations are as indicated in table S2. Clusters are labeled 1, 2 and 4-6, corresponding to the clusters in figure S2A.

Figure S2C: Phylogenetic tree of CDF proteins. Protein abbreviations are as indicated in table S3. Clusters are labeled 1-11.

Figure S3: Average hydropathy (dark line, top) and average similarity (light line, bottom) plots of Stim protein homologues (see table S2). This plot was generated with the AveHas program (Zhai and Saier, 2001) based on the multiple alignment shown in figure S1B.

Figure S4: Multiple alignment of eleven Orai homologues with eleven CDF homologues. The two proteins used as query sequences in BLAST searches were the C. elegans Orai homologue (NP_497230) and the Pyrococcus furiosus CDF homologue (AAL80682). These two proteins plus 10 homologues retrieved from each search with good scores, each from a different species, were included in the ClustalX multiple alignment (see Methods section). With 116 positions shown, there are three positions where only identities (a single residue) are present (asterisks), eighteen positions where only close similarities are present (colons), and eleven positions where only more distant similarities are present (dots) as defined by the ClustalX program (see Methods). Thus, 32 positions, or 28% of all positions, show identities and similarities in all twenty two proteins included in the alignment. The species name, the protein genbank ID#, the starting residue number, the sequence included in the alignment, and the final residue number for each protein are shown in the figure.

Tables:

Table S1: List of Orai protein sequences from the CRAC-C family included in this study.

Table S2: List of Stim protein sequences from the CRAC-C family included in this study.

Table S3: List of Cation Diffusion Facilitator sequences included in this study.

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