VIDEO

15. Co-localized activation of Ras and PI3K at the leading edge.

Co-localized activation of Ras and PI3K at the leading edge. The movie shows the spatial and temporal co-localization of activated Ras (Ras-GTP) and PI3K at the leading edge of wild-type chemotaxing cells. Cells are co-expressing a reporter for activated Ras (GFP-RBD, Ras binding domain) and one (RFP-for PI(3,4,5)P3, the product of PI3K. Green- GFP-RBD; Red, RFP-PHcrac; Yellow, merge of the two images. Contemporaneous image collection was achieved using a beam splitter.