12. Localization of F-actin in ARK1 null cells chemotaxing towards cAMP visualized using ABP-GFP

Time-lapse video microscopy of ARK1 null cells expressing a GFP fusion of the F-actin binding domain from ABP120 (a gift from David Knecht, Univ. of Conn.). ARK1, an ankyrin-repeat containing kinase is required for proper chemotaxis. The GFP fusion of the actin binding domain from ABP120 is used to visualize F-actin in vivo. The movie shows the localization of the F-actin in these cells. Note the very broad leading edge and multiple pseudopodia in many of the ARK1 null cells that are not usually seen in wild-type cells. B. Sun, Firtel lab.