VIDEO

14. Translocation of the PhdA PH-domain-containing protein

Translocation of the PhdA PH-domain-containing protein to the leading edge in response to a directional signal. PhdA is required for proper chemotaxis in Dictyostelium. The movie demonstrates that PhdA rapidly translocates to the leading edge of chemotaxing amoebae in response to a micropipette containing the chemoattractant cAMP. When the micropipette is moved to a new position relative to the cell, the PhdA-GFP is lost from the old site and the old pseudopod rapidly retracts. PhdA now localizes to a position proximal to the new cAMP source and forms a new pseudopod. S. Funamoto, Firtel lab.