VIDEO

9. Localization of the Akt/PKB PH domain to the leading edge of chemotaxing cells

Time-lapse video microscopy of the PH domain from the serine/threonine kinase Akt/PKB fused to GFP in cells chemotaxing to cAMP. The position of the micropipette is in the upper left corner of the frame (not visible). Note localization of the PH domain to the leading edge of many of the cells. R. Meili and C. Ellsworth, Firtel lab.