VIDEO

10. Localization of a PH domain-containing protein (phdA) to the plasma membrane in response to changes in the direction of the cAMP source

Time-lapse video movie of a GFP fusion of a novel PH domain-containing protein (phdA), which is required for proper chemotaxis, to the plasma membrane in response to directional changes in the cAMP source. The position of the micropipette is indicated by a star. Note that as the position of the micropipette is moved, the GFP fusion localizes to a position in the plasma membrane closest to the cAMP source and is lost from the membrane at its previous site. In some cases, a new pseudopod extends shortly after membrane localization of the protein and retracts as the micropipette containing cAMP is moved. S. Funamoto, Firtel lab.