Publications 1992 - 1997

Hadwiger, J.A. and R.A. Firtel (1992).  Analysis of G-alpha-4, a G protein subunit required for multicellular development in Dictyostelium. Genes Devel. 6:38-49.

Esch, R.K., P.K. Howard and R.A. Firtel (1992).  Regulation of the Dictyostelium cAMP-induced, prestalk-specific DdrasD gene: identification of cis-acting elements.  Nucl. Acids Res. 20:1325-1332.

Cubitt, A.B. and R.A. Firtel (1992).  Characterization of phospholipase activity in Dictyostelium discoideum. Identification of a Ca2+-dependent phosphoinositidase-specific phospholipase C.  Biochem. J. 283:371-378.

Powell, J.A., J. Galindo and R.A. Firtel (1992).  A negative transcriptional control region of a developmentally-regulated gene co-localizes with the origin of replication of an endogenous plasmid in Dictyostelium.  Nucl. Acids Res. 20:2795-2802.

Ceccarelli, A., H.J. Mahbubani, R. Insall, G. Schnitzler, R.A. Firtel and J.G. Williams (1992).  A G-rich sequence element common to Dictyostelium genes which differ radically in their patterns of expression.  Devel. Biol. 152:188-193.

Okaichi, K., A.B. Cubitt, G. Pitt and R.A. Firtel (1992).  Amino acid substitutions in the Dictyostelium G-alpha subunit G-alpha-2 produce dominant negative phenotypes and inhibit the activation of adenylyl cyclase, guanylyl cyclase, and phospholipase C.  Mol. Biol. Cell 3:735-747.

Howard, P., B. Sefton and R.A. Firtel (1992).  Analysis of a spatially regulated phosphotyrosine phosphatase identifies tyrosine phosphorylation as a key regulatory pathway in Dictyostelium.  Cell 71:637-647.

Mann, S.K.O., W.M. Yonemoto, S.S. Taylor and R.A. Firtel (1992).  DdPK3, which plays essential roles during Dictyostelium development, encodes  the catalytic subunit of cAMP-dependent protein kinase.  Proc. Natl. Acad. Sci. USA 89:10701-10705.

Howard, P.K., B.M. Sefton and R.A. Firtel (1993).  Tyrosine phosphorylation of actin in Dictyostelium associated with cell shape changes.  Science 259:241-244. Abstract

Powell, J.A., G.R. Schnitzler, J. Hadwiger, P. Howard, R.K. Esch, A.B. Cubitt, K. Okaichi, C. Gaskins, S.K.O. Mann and R.A. Firtel (1993). Spatial and temporal regulation of Dictyostelium development through signal transduction pathways.  In: Evolutionary Conservation of Developmental Mechanisms, Wiley-Liss, Inc., pp. 159-184.

Powell-Coffman, J.A. and R.A. Firtel (1993).  Cellular dedifferentiation and spore germination in Dictyostelium may utilize similar regulatory pathways. BioEssays 15:131-133.

Kikkawa, U., S.K.O. Mann, R.A. Firtel and T. Hunter (1993).  Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle.  Mol. Cell. Biol. 12:5711-5723.

Cell-permeable non-hydrolyzable cAMP derivatives as tools for analysis of signalingSchaap, P., M. Van Ments-Cohen, R.D.M. Soede, R. Brandt, R.A. Firtel, W. Dostmann, H.-G. Genieser, B. Jastorff and P.J.M. Van Haastert (1993). Pathways controlling gene regulation in Dictyostelium.  J. Biol. Chem. 268:6323-6331.

Cubitt, A.B., F. Carrel, S. Dharmawardhane, C. Gaskins, J. Hadwiger, P. Howard, S.K.O. Mann, K. Okaichi, K. Zhou and R.A. Firtel (1993).  Molecular genetic analysis of signal transduction pathways controlling multicellular development in Dictyostelium.  Cold Spring Harbor Symp. Quant. Biol. LVII:177-192.

Cubitt, A.B., S. Dharmawardhane and R.A. Firtel (1993).  Developmentally regulated changes in 1,2-diacylglycerol in Dictyostelium: Regulation by light and G proteins.  J. Biol. Chem. 268:17431-17439.

Schatzle, J., J. Bush, S. Dharmawardhane, R.A. Firtel, R.H. Gomer and J. Cardelli (1993).  Characterization of the signal transduction pathways and cis-acting DNA sequence responsible for the transcriptional induction during growth and development of the lysosomal a-mannosidase gene in Dictyostelium discoideum. J. Biol. Chem. 268:19632-19639.

Stober-Grasser, U., B. Brydolf, X. Bin, F. Grasser, R.A. Firtel and J.S. Lipsick (1993).  The myb DNA-binding domain is highly conserved in Dictyostelium discoideum.  Oncogene 7:589-596.

Esch, R.K., G. Weeks and R.A. Firtel (1993).  Regulation and function of two developmentally regulated ras genes in Dictyostelium.  In: The Ras Superfamily of GTPases (eds. J.C. Lacal and F. McCormick), FL: CRC Press, pp. 173-186.

Mann, S.K.O. and R.A. Firtel (1993).  cAMP-dependent protein kinase differentially regulates prestalk and prespore differentiation during Dictyostelium development.  Development 119:135-146.  Abstract

Carrel, F., S. Dharmawardhane, A.M. Clark, J.A. Powell-Coffman and R.A. Firtel (1994).  Spatial and temporal expression of the Dictyostelium discoideum subunit G-alpha-2: Expression of a dominant negative protein inhibits proper prestalk to stalk differentiation.  Mol. Biol. Cell 5:7-16.  Abstract

Schnitzler, G.R., W.H. Fischer and R.A. Firtel (1994). Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium.  Genes Devel. 8:502-514.  Abstract

Wu, L., C. Gaskins, R. Gundersen, J.A. Hadwiger, R.L. Johnson, G.S. Pitt, R.A. Firtel and P.N. Devreotes (1994).  Signal transduction by G-proteins in Dictyostelium discoideum.  In: Handbook of Experimental Pharmacology, Vol. 108/II GTPases in Biology II (eds. B. Dickey and L. Birnbaumer), Berlin, Germany: Springer-Verlag Publication, pp. 335-349.

Dynes, J.L., A.M. Clark, G. Shaulsky, A. Kuspa, W.F. Loomis and R.A. Firtel (1994).  LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium.  Genes Devel. 8:948-958.  Abstract

Wu, L., C. Gaskins, K. Zhou, R.A. Firtel and P.N. Devreotes (1994). Cloning and targeted mutations of G-alpha-7 and G-alpha-8, two developmentally regulated G protein a-subunit genes in Dictyostelium.  Mol. Biol. Cell 5:691-702.  Abstract

Powell-Coffman J.A. and R.A. Firtel (1994). Characterization of a novel Dictyostelium discoideum prespore-specific gene, PspB, reveals conserved regulatory sequences.  Development 120:1601-1611.  Abstract

Powell-Coffman, J.A., G.R. Schnitzler and R.A. Firtel (1994).  A GBF-binding site and a novel AT element define the minimal sequences sufficient to direct prespore-specific expression in Dictyostelium.  Mol. Cell. Biol. 14:5840-5849.  Abstract

Howard, P.K., M. Gamper, T. Hunter and R.A. Firtel (1994). Regulation by protein-tyrosine phosphatase PTP2 is distinct from that by PTP1 during Dictyostelium growth and development.  Mol. Cell. Biol. 14:5154-5164.  Abstract

Gaskins, C., M. Maeda and R.A. Firtel (1994). Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.  Mol. Cell. Biol. 14:6996-7012.  Abstract

Hadwiger, J.A., S. Lee and R.A. Firtel (1994).  The G-alpha subunit G-alpha-4 couples to pterin receptors and identifies a signaling pathway that is essential for multicellular development in Dictyostelium.  Proc. Natl. Acad. Sci. USA 91:10566-10570. Abstract

Mann, S.K., D.L. Richardson, S. Lee, A.R. Kimmel and R.A. Firtel (1994).  Expression of cAMP-dependent protein kinase in prespore cells is sufficient to induce spore cell differentiation in Dictyostelium.  Proc. Natl. Acad. Sci. USA 91:10561-10565. Abstract

Mann, S.K.O., P.N. Devreotes, S. Eliott, K. Jermyn, A. Kuspa, M. Fechheimer, R. Furukawa, C.A. Parent, J. Segall, G. Shaulsky, P.H. Vardy, J. Williams, K. Williams and R.A. Firtel (1994).  Cell biological, molecular genetic, and biochemical methods to examine Dictyostelium.  In: Cell Biology: A Laboratory Handbook (ed. J. Celis), Academic Press, pp. 412-451.

Hori, R. and R.A. Firtel (1994).  Identification and characterization of multiple A/T-rich cis-acting elements that control expression from the Dictyostelium actin promoters: The Dictyostelium actin upstream activating sequence confers growth-phase expression and has enhancer-like properties.  Nucl. Acids Res. 22:5099-5111.

Dharmawardhane, S., A.B. Cubitt and R.A. Firtel (1994).  Regulatory role of G-alpha-1 subunit in controlling cellular morphogenesis in Dictyostelium. Development 120:3549-3561. Abstract

Segall, J.E., A. Kuspa, G. Shaulsky, M. Ecke, M. Maeda, C. Gaskins, R.A. Firtel and W.F. Loomis (1995).  A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium.  J. Cell Biol. 128:405-413.

Cao, J.-G. and R.A. Firtel (1995). Growth and developmental functions of a human immunodeficiency virus Tat-binding protein/26S protease subunit homolog from Dictyostelium discoideum.  Mol. Cell. Biol. 15:1725-1736.

Briscoe, C. and R.A. Firtel (1995).  A kinase for cell-fate determination? GSK-3, a ubiquitous kinase regulated by tyrosine phosphorylation, controls cell-fate decisions in both Drosophila and Dictyostelium; genetic analysis of its interactions with other signaling pathways is now possible.  Curr. Biol. 5:228-231.

Gamper, M., P.K. Howard, T. Hunter and R.A. Firtel (1995).  Protein tyrosine phosphatases in Dictyostelium discoideum.  Adv. Prot. Phosphatases 9:25-49.

Schnitzler, G.R., C. Briscoe, J.M. Brown and R.A. Firtel (1995).  Serpentine cAMP receptors may act though a G protein-independent pathway to induce postaggregative development in Dictyostelium.  Cell 81:737-745.  Abstract

Firtel, R.A. (1995).  Integration of signaling information in controlling cell-fate decisions in Dictyostelium.  Genes Devel. 9:1427-1444.

Cubitt, A.B., R.A. Firtel, G. Fischer, L.F. Jaffe and A.L. Miller (1995).  Patterns of free calcium in multicellular stages of Dictyostelium expressing jellyfish apoaequorin. Development 121:2291-2301.  Full text  Abstract

Gaskins, C., A.M. Clark, L. Aubry, J.E. Segall and R.A. Firtel (1996).  The Dictyostelium MAP kinase ERK2 regulates multiple, independent developmental pathways. Genes Devel. 10:118-128.  Abstract

Maeda, M., L. Aubry, R. Insall, C. Gaskins, P. Devreotes and R. Firtel (1996).  Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium.  J. Biol. Chem. 271:3351-3354.  Full text  Abstract

Gamper, M., P.K. Howard and R.A. Firtel (1996).  Multiple roles of the novel protein tyrosine phosphatase PTP3 during Dictyostelium growth and development.  Mol. Cell. Biol. 16:2431-2444.  Abstract

Zhou, K., K. Takegawa, S.D. Emr and R.A. Firtel (1996).  A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: Biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development.  Mol. Cell. Biol. 15:5645-5656.  Abstract

Hadwiger, J.A., K. Natarajan and R.A. Firtel (1996).  Mutations in the Dictyostelium heterotrimeric G protein alphasubunit G-alpha-5 alter the kinetics of tip morphogenesis.  Development 122:1215-1224.  Full text  Abstract

Firtel, R.A. (1996).  Interacting signaling pathways controlling multicellular development in Dictyostelium.  Curr. Opin. Genet. Devel. 6:545-554.

Aubry, L., M. Maeda, R. Insall, P.N. Devreotes and R.A. Firtel (1997).  The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by Ras and cAMP-dependent protein kinase (PKA) and mediates PKA function.  J. Biol. Chem. (Communication) 272:3883-3886. Full text  Abstract

Mann, S.K.O., P.N. Devreotes, S. Eliott, K. Jermyn, A. Kuspa, M. Fechheimer, R. Furukawa, C.A. Parent, J. Segall, G. Shaulsky, P.H. Vardy, J. Williams, K.L. Williams and R.A. Firtel (1998).  Cell biological, molecular genetic, and biochemical methods used to examine Dictyostelium.  In: Cell Biology: A Laboratory Handbook, Second Edition, Volume 1 (ed. J. Celis), Academic Press, pp. 431-465.

Mann, S.K.O., J.M. Brown, C. Briscoe, C. Parent, G. Pitt, P.N. Devreotes and R.A. Firtel (1997).  Role of cAMP-dependent protein kinase in controlling aggregation and post-aggregative development in Dictyostelium.  Devel. Biol. 183:208-221.  Full text  Abstract

Lee, S., R. Escalante and R.A. Firtel (1997).  A Ras GAP is essential for cytokinesis and spatial patterning in Dictyostelium.  Development 124:983-996.  Full text  Abstract

Ma, H., M. Gamper, C. Parent and R.A. Firtel (1997).  The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase.  EMBO J. 16:4317-4332.  Full text  Abstract

Buczynski, G, B. Grove, A. Nomura, M. Kleve, J. Bush, R.A. Firtel and J. Cardelli (1997).  Inactivation of two Dictyostelium discoideum genes, DdPIK1 and DdPIK2, encoding proteins related to mammalian phosphatidylinositide 3-kinases, results in defects in endocytosis, lysosome to postlysosome transport, and actin cytoskeleton organization.  J. Cell Biol. 136:1271-1286.  Full text

Maeda, M. and R.A. Firtel (1997).  Activation of the mitogen-activated protein kinase ERK2 by the chemoattractant folic acid in Dictyostelium.  J. Biol. Chem. 272:3690-23695.  Full text  Abstract

Clark, A., A. Nomura , S. Mohanty and R.A. Firtel (1997).  A ubiquitin-conjugating enzyme is essential for developmental transitions in Dictyostelium.  Mol. Biol. Cell 8:1989-2002.  Full text  Abstract

Brown, J.M., C. Briscoe and R.A. Firtel (1997).  Control of transcriptional regulation by signal transduction pathways in Dictyostelium during multicellular development.  In: Dictyostelium--A Model System for Cell and Developmental Biology (eds Y. Maeda, K. Inouye and I. Takeuchi), Universial Academy Press, Inc., Tokyo, pp. 245-265.

Rietdorf, J., F. Siegert, S. Dharmawardhane, R.A. Firtel and C.J. Weijer (1997).  Analysis of cell movement and signalling during ring formation in an activated Ga1 mutant of Dictyostelium discoideum that is defective in prestalk zone formation.  Devel. Biol. 181:79-90.  Full text