Multiplex Fluorescent mRNA In Situ Hybridization

9/26/03: This site is under construction, but is nearly complete. It presents information on how to do multiplex fluorescent mRNA in situ hybridization on Drosophila embryos. Questions, suggestions, or comments, please e-mail me at The following links provide protocols, images, and general information on this project:

Prepared by Dave Kosman (Ethan Bier and Bill McGinnis labs, UC San Diego)
Protocols developed in collaboration with Mieko Mizutani and Greg Cox
Project supported by NSF IBN0120728